Article
Global gene disruption in human cells to assign genes to phenotypes by deep sequencing.
Nature biotechnology - 29 May 2011
Carette Jan E, Guimaraes Carla P, Wuethrich Irene, Blomen Vincent A, Varadarajan Malini, Sun Chong, Bell George, Yuan Bingbing, Muellner Markus K, Nijman Sebastian M, Ploegh Hidde L, Brummelkamp Thijn R
Abstract excerpt
Insertional mutagenesis in a haploid background can disrupt gene function. We extend our earlier work by using a retroviral gene-trap vector to generate insertions in >98% of the genes expressed in a human cancer cell line that is haploid for all but one of its chromosomes. We apply phenotypic interrogation via tag sequencing (PhITSeq) to examine millions of mutant alleles through selection and parallel...
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