Characterization of poliovirus clones containing lethal and nonlethal mutations in the genome-linked protein VPg.

doi:10.1128/JVI.64.6.2967-2975.1990

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Article

Characterization of poliovirus clones containing lethal and nonlethal mutations in the genome-linked protein VPg.

Journal of virology - 1 Jun 1990

Reuer Q, Kuhn R J, Wimmer E

Abstract excerpt

Viral RNA synthesis was assayed in HeLa cells transfected with nonviable poliovirus RNA mutated in the genome-linked protein VPg-coding region. The transfecting RNA was transcribed in vitro from full-length poliovirus type 1 (Mahoney) cDNA containing a VPg mutagenesis cartridge. Hybridization experiments using ribonucleotide probes specific for the 3' end of positive- and negative-sense poliovirus RNA indicated...

Topics

Amino Acid Sequence
Base Sequence
Cloning, Molecular
Enterovirus B, Human
Genes, Lethal
Genes, Viral
HeLa Cells
Humans
Molecular Sequence Data
Mutation
Nucleic Acid Hybridization

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Poliovirus

RNA Probes

RNA, Viral

Sequence Homology, Nucleic Acid

Transcription, Genetic

Viral Core Proteins

Source

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Identifiers

PMID: 2159557
DOI: 10.1128/JVI.64.6.2967-2975.1990
PMCID: PMC249481

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2159557-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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