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Article

Infectious RNA transcripts derived from cloned cDNA of papaya mosaic virus: effect of mutations to the capsid and polymerase proteins.

The Journal of general virology - 1 Jun 1993

Sit T L, AbouHaidar M G

Abstract excerpt

Genomic length cDNAs of papaya mosaic virus (PMV) RNA were generated utilizing reverse transcriptase (RNase H-) for first strand synthesis, Sequenase for second strand synthesis and primers specific for the 5' and 3' termini of the viral genome. These cDNAs were cloned into plasmid pUC18 and infectious RNA transcripts were synthesized in vitro from a bacteriophage T7 RNA polymerase promoter incorporated into the...

Topics

Base Sequence
Capsid
Cloning, Molecular
DNA, Viral
Fruit
Molecular Sequence Data
Mosaic Viruses
Mutation
RNA, Messenger
RNA, Viral
RNA-Directed DNA Polymerase

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Transcription, Genetic

Virulence

Source

Identifiers

PMID: 7685373
DOI: 10.1099/0022-1317-74-6-1133

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7685373-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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