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Article

Fluorescence and NMR investigations on the ligand binding properties of adenylate kinases.

Biochemistry - 14 Aug 1990

Reinstein J, Vetter I R, Schlichting I, Rösch P, Wittinghofer A, Goody R S

Abstract excerpt

A new system for measurement of affinities of adenylate kinases (AK) for substrates and inhibitors is presented. This system is based on the use of the fluorescent ligand alpha,omega-di[(3' or 2')-O-(N-methylanthraniloyl)adenosine-5'] pentaphosphate (mAP5Am), which is an analogue of the bisubstrate inhibitor diadenosine pentaphosphate (AP5A). It allows the determination of dissociation constants for any ligand in...

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PMID: 2223775
DOI: 10.1021/bi00484a013

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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