Second gene expression in bicistronic constructs using short synthetic intercistrons and viral IRES sequences.

doi:10.1016/s0378-1119(98)00453-3

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Article

Second gene expression in bicistronic constructs using short synthetic intercistrons and viral IRES sequences.

Gene - 19 Nov 1998

Havenga M J, Vogels R, Braakman E, Kroos N, Valerio D, Hagenbeek A, van Es H H

Abstract excerpt

In this study, we describe the efficiency of second gene translation in bicistronic constructs containing either a short (36bp) synthetic intercistron or known internal ribosomal entry sites (IRES). Experiments were performed using two different gene combinations: Herpes simplex virus-thymidine k...

Topics

Animals
Cell Line
DNA
Drug Resistance
Gene Expression
Genes
Genes, Viral
Glucosylceramidase
Humans
Methotrexate
Mutation
Neomycin
Plasmids
Protein Biosynthesis
RNA, Messenger
Rats
Retroviridae
Simplexvirus

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