Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Second gene expression in bicistronic constructs using short synthetic intercistrons and viral IRES sequences.

Gene - 19 Nov 1998

Havenga M J, Vogels R, Braakman E, Kroos N, Valerio D, Hagenbeek A, van Es H H

Abstract excerpt

In this study, we describe the efficiency of second gene translation in bicistronic constructs containing either a short (36bp) synthetic intercistron or known internal ribosomal entry sites (IRES). Experiments were performed using two different gene combinations: Herpes simplex virus-thymidine kinase (HSV-TK) and neomycine (NEO) or human glucocerebrosidase (hGC) and a methotrexate (MTX) resistant mutant...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Line
Share to DNA
Share to Gene Expression

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Mutation

Share to Plasmids

Share to RNA, Messenger

Source

Identifiers

PMID: 9831667
DOI: 10.1016/s0378-1119(98)00453-3

Genes

DHFR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Second gene expression in bicistronic constructs using short synthetic intercistrons and viral IRES sequences.PMID 9831667Read ↗

Improved vectors for stable expression of foreign genes in mammalian cells by use of the untranslated leader sequence from EMC virusSemantic proximity Read ↗

Multigene lentiviral vectors based on differential splicing and translational control.Semantic proximity Read ↗

Production and Application of Multicistronic Constructs for Various Human Disease TherapiesSemantic proximity Read ↗

Improved co-expression of multiple genes in vectors containing internal ribosome entry sites (IRESes) from human genesSemantic proximity Read ↗

Polycistronic Viral VectorsSemantic proximity Read ↗

Retroviral vectors containing putative internal ribosome entry sites: development of a polycistronic gene transfer system and applications to human gene therapySemantic proximity Read ↗

IRES Bicistronic Expression Vectors for Efficient Creation of Stable Mammalian Cell LinesSemantic proximity Read ↗

Internal initiation of translation in retroviral vectors carrying picornavirus 5' nontranslated regionsSemantic proximity Read ↗

The optimal use of IRES (internal ribosome entry site) in expression vectorsSemantic proximity Read ↗

An unusual internal ribosome entry site in the herpes simplex virus thymidine kinase geneSemantic proximity Read ↗

Inducible, High-Level Production of Infectious Murine Leukemia Retroviral Vector Particles Pseudotyped with Vesicular Stomatitis Virus G Envelope ProteinSemantic proximity Read ↗

Co-Expression of Human Adenosine Deaminase and Multidrug Resistance Using a Bicistronic Retroviral VectorSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.