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Article

Mutagenesis of Bordetella pertussis with transposon Tn5tac1: conditional expression of virulence-associated genes.

Journal of bacteriology - 1 Apr 1990

Cookson B T, Berg D E, Goldman W E

Abstract excerpt

The Tn5tac1 transposon contains a strong outward-facing promoter, Ptac, a lacI repressor gene, and a selectable Kanr gene. Transcription from Ptac is repressed by the lacI protein unless an inducer (isopropyl-beta-D-thiogalactopyranoside [IPTG]) is present. Thus, Tn5tac1 generates insertion mutations in Escherichia coli with conditional phenotypes because it is polar on distal gene expression when IPTG is absent...

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Identifiers

PMID: 2156797
DOI: 10.1128/jb.172.4.1681-1687.1990
PMCID: PMC208656

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