Article
High frequency of independent IS50 transposition during Tn5 mutagenesis of Bordetella pertussis virulence-associated genes.
Letters in applied microbiology - 1 Oct 1992
Ward M J, Duggleby C J, Parton R, Coote J G
Abstract excerpt
Transposon Tn5-generated mutants of Bordetella pertussis were selected on the basis of their inability to bind the dye Congo red (Crb-). No mutants which were solely Crb- were found. Ten mutants were phenotypically equivalent to previously described strains with mutations in the virulence regulatory (bvg) locus and failed to express a range of virulence-associated factors. Two of these mutants were shown to have...
Topics
- Bordetella pertussis
- DNA Transposable Elements
- Gene Frequency
- Genes, Bacterial
- Mutagenesis, Insertional
- Phenotype
- Virulence