Article
Mutants in the ptlA-H genes of Bordetella pertussis are deficient for pertussis toxin secretion.
FEMS microbiology letters - 15 Oct 1999
Craig-Mylius K A, Weiss A A
Abstract excerpt
The nine ptl genes (A-I) are required for efficient secretion of pertussis toxin past the outer membrane. Mutations were made in ptlA-H by filling in unique restriction sites, generating in-frame deletions, or inserting a FLAG epitope tag. The mutations were cloned into a suicide shuttle plasmid containing the ptxptl operon and introduced into the adenylate cyclase locus of the chromosome of a Bordetella...
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