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Article

Properties of new Escherichia coli Hfr strains constructed by integration of pSC101-derived conjugative plasmids.

Journal of bacteriology - 1 Mar 1990

François V, Conter A, Louarn J M

Abstract excerpt

Conjugative temperature-sensitive plasmids were derived from pSC101. These plasmids are useful in genetic analysis for two reasons: (i) they render possible the construction of new Hfr lines by plasmid integration at predetermined chromosomal loci via Tn10 inverse transposition, and (ii) the Hfr characters are transducible via bacteriophage P1. We also showed that replication from pSC101 origin is deleterious for...

Topics

Conjugation, Genetic
DNA Transposable Elements
Escherichia coli
Genotype
Mutation
Phenotype
Plasmids
Temperature

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 2155201
DOI: 10.1128/jb.172.3.1436-1440.1990
PMCID: PMC208617

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2155201-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Properties of new Escherichia coli Hfr strains constructed by integration of pSC101-derived conjugative plasmids.PMID 2155201Read ↗

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