Article
Functional analysis of the response regulator DegU in Bacillus megaterium DSM319 and comparative secretome analysis of degSU mutants.
Applied microbiology and biotechnology - 1 Aug 2011
Borgmeier Claudia, Voigt Birgit, Hecker Michael, Meinhardt Friedhelm
Abstract excerpt
We functionally analysed the two-component regulatory system DegSU (historically SacU) in Bacillus megaterium DSM319 by generating a genetic knock out as well as a sacU32 mutation. The latter--known to cause a hypersecretion phenotype in Bacillus subtilis--had no influence on extracellular protease and amylase activity in B. megaterium. Since the B. megaterium DegU complemented a Bacillus licheniformis ∆degSU...
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