Isolation and expansion of regionally defined human glioblastoma cells in vitro.

Abstract excerpt

This unit describes a protocol for the surgical collection, isolation, and expansion of regionally defined human glioblastoma multiforme (GBM)-derived cells. Given the important role played by microenvironmental hypoxia in defining cell phenotype and molecular signaling activation, it is important to distinguish between tumor tissues that were originally localized within the partially necrotic tumor core and...

Topics

• Cell Culture Techniques
• Cell Proliferation
• Cell Separation
• Glioblastoma
• Humans
• Magnetic Resonance Imaging
• Phenotype
• Signal Transduction