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Article

Synthesis of hepadnavirus particles that contain replication-defective duck hepatitis B virus genomes in cultured HuH7 cells.

Journal of virology - 1 Feb 1990

Horwich A L, Furtak K, Pugh J, Summers J

Abstract excerpt

To evaluate the possibility of producing transducible replication-defective hepadnaviruses, cloned mutant duck hepatitis B virus genomes were tested both for virus antigen production and viral DNA synthesis following transfection into the human hepatoma cell line HuH7. Deletion of a cis-acting 12-nucleotide sequence implicated in viral DNA synthesis, direct repeat 1 (DR1), resulted in the loss of ability to...

Topics

Animals
Carcinoma, Hepatocellular
Cell Line
Cells, Cultured
DNA Replication
Defective Viruses
Ducks
Genes, Viral
Hepatitis B Virus, Duck
Hepatitis B virus
Humans

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Liver

Liver Neoplasms

Mutation

Transfection

Virus Replication

Source

Open in PubMed Open full text

Identifiers

PMID: 2153230
DOI: 10.1128/JVI.64.2.642-650.1990
PMCID: PMC249155

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2153230-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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