Article
Launching the yeast 23S RNA Narnavirus shows 5' and 3' cis-acting signals for replication.
Proceedings of the National Academy of Sciences of the United States of America - 4 Mar 2003
Esteban Rosa, Fujimura Tsutomu
Abstract excerpt
Narnavirus 23S RNA is a persistent positive-stranded RNA virus found in yeast Saccharomyces cerevisiae. The viral genome (2.9 kb) only encodes its RNA-dependent RNA polymerase, p104. Here we report the generation of 23S RNA virus, with high frequency, from a vector containing the entire viral cDNA sequence. When the conserved GDD (Gly-Asp-Asp) motif of RNA-dependent RNA polymerase was modified, the vector failed...
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