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Rescue of a segmented negative-strand RNA virus entirely from cloned complementary DNAs

24 Dec 1996

Abstract excerpt

We provide the first report, to our knowledge, of a helper-independent system for rescuing a segmented, negative-strand RNA genome virus entirely from cloned cDNAs. Plasmids were constructed containing full-length cDNA copies of the three Bunyamwera bunyavirus RNA genome segments flanked by bacteriophage T7 promoter and hepatitis delta virus ribozyme sequences. When cells expressing both bacteriophage T7 RNA...

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PMID: 8986823
DOI: 10.1073/pnas.93.26.15400

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