Article
Glycerol facilitator of Escherichia coli: cloning of glpF and identification of the glpF product.
Journal of bacteriology - 1 Jan 1990
Sweet G, Gandor C, Voegele R, Wittekindt N, Beuerle J, Truniger V, Lin E C, Boos W
Abstract excerpt
The glycerol facilitator is known as the only example of a transport protein that catalyzes facilitated diffusion across the Escherichia coli inner membrane. Here we show that the gene encoding the facilitator, glpF, is the first gene in an operon with glpK, encoding glycerol kinase, at 88 min of the E. coli chromosome. The operon is transcribed counterclockwise. We cloned the glpF gene, demonstrated that it...
Topics
- Bacterial Proteins
- Carrier Proteins
- Cloning, Molecular
- Escherichia coli
- Glycerol
- Glycerol Kinase
- Mutation
- Operon
- Phosphotransferases
- Plasmids
- Promoter Regions, Genetic