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Article

Genetic analysis of the genes involved in synthesis of the lipopolysaccharide core in Escherichia coli K-12: three operons in the rfa locus.

Journal of bacteriology - 1 May 1992

Roncero C, Casadaban M J

Abstract excerpt

The region of the Escherichia coli K-12 chromosome encoding the enzymes responsible for the synthesis of responsible for the synthesis of the lipopolysaccharide (LPS) core has been cloned in vivo by using a mini-Mu vector. This region, formerly known as the rfa locus, comprises 18 kb of DNA betwe...

Topics

Chromosome Mapping
Cloning, Molecular
DNA Mutational Analysis
Escherichia coli
Genetic Complementation Test
Lac Operon
Lipopolysaccharides
Multigene Family
Operon
Phenotype
Polysaccharides, Bacterial
Reading Frames

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Recombinant Fusion Proteins

Recombination, Genetic

Salmonella typhimurium

beta-Galactosidase

Source

Open in PubMed Open full text

Identifiers

PMID: 1577693
DOI: 10.1128/jb.174.10.3250-3260.1992
PMCID: PMC205993

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-1577693-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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Genetic analysis of the genes involved in synthesis of the lipopolysaccharide core in Escherichia coli K-12: three operons in the rfa locus.PMID 1577693Read ↗

Identification and sequences of the lipopolysaccharide core biosynthetic genes rfaQ, rfaP, and rfaG of Escherichia coli K-12.Semantic proximity Read ↗

Structures of the rfaB, rfaI, rfaJ, and rfaS genes of Escherichia coli K-12 and their roles in assembly of the lipopolysaccharide core.Semantic proximity Read ↗

Genetic analysis of lipopolysaccharide core biosynthesis by Escherichia coli K-12: insertion mutagenesis of the rfa locus.Semantic proximity Read ↗

Cloning, characterization, and DNA sequence of the rfaLK region for lipopolysaccharide synthesis in Salmonella typhimurium LT2.Semantic proximity Read ↗

Identification of bacteriophage K20 binding regions of OmpF and lipopolysaccharide in Escherichia coli K-12.Semantic proximity Read ↗

Genes for TDP-rhamnose synthesis affect the pattern of lipopolysaccharide heterogeneity in Escherichia coli K-12.Semantic proximity Read ↗

Cloning, expression, and characterization of the Escherichia coli K-12 rfaD gene.Semantic proximity Read ↗

Effect of lipopolysaccharide core synthesis mutations on the production of Vibrio cholerae O-antigen in Escherichia coli K-12.Semantic proximity Read ↗

Molecular cloning of the rfb region of Klebsiella pneumoniae serotype O1:K20: the rfb gene cluster is responsible for synthesis of the D-galactan I O polysaccharide.Semantic proximity Read ↗

Lipopolysaccharide with an altered O-antigen produced in Escherichia coli K-12 harbouring mutated, cloned Shigella flexneri rfb genes.Semantic proximity Read ↗

A novel strategy to isolate cell-envelope mutants resistant to phage infection: bacteriophage mEp213 requires lipopolysaccharides in addition to FhuA to enter Escherichia coli K-12.Semantic proximity Read ↗

Molecular cloning and genetic analysis of the rfb region from Shigella flexneri type 6 in Escherichia coli K-12.Semantic proximity Read ↗

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