Article
Mutational analysis of the Escherichia coli glpFK region with Tn5 mutagenesis and the polymerase chain reaction.
Journal of bacteriology - 1 Oct 1990
Lupski J R, Zhang Y H, Rieger M, Minter M, Hsu B, Ooi B G, Koeuth T, McCabe E R
Abstract excerpt
Transposon Tn5 mutagenesis of the Escherichia coli chromosome was used to isolate 21 independent insertion mutations conferring an altered colony color phenotype on MacConkey-glycerol plates. The polymerase chain reaction was used to map 16 of these Tn5 insertions within the glpFK region at 88 mi...
Topics
- Aquaporins
- Bacterial Outer Membrane Proteins
- Chromosomes, Bacterial
- DNA Transposable Elements
- DNA, Bacterial
- Escherichia coli
- Escherichia coli Proteins
- Glycerol
- Kinetics
- Membrane Proteins
- Mutation
- Operon
- Phenotype
- Polymerase Chain Reaction
- Restriction Mapping