Characterization of the Zymomonas mobilis glucose facilitator gene product (glf) in recombinant Escherichia coli: examination of transport mechanism, kinetics and the role of glucokinase in glucose transport.

Abstract excerpt

Zymomonas mobilis is known to transport glucose by a facilitated diffusion process. A putative glucose facilitator gene (glf), closely related to a large family of glucose transporters, is located in a cluster of genes that code for enzymes of glucose metabolism. The Z. mobilis glf gene is able to complement glucose transport in an Escherichia coli strain that is defective in native glucose transport and...

Topics

• Bacterial Proteins
• Biological Transport
• DNA, Recombinant
• Escherichia coli
• Gene Expression Regulation, Bacterial
• Genotype
• Glucokinase
• Glucose
• Isopropyl Thiogalactoside
• Kinetics