Article
Genetic mapping of the obligate methylotroph Methylobacillus flagellatum: characteristics of prime plasmids and mapping of the chromosome in time-of-entry units.
Journal of bacteriology - 1 May 1990
Tsygankov Y D, Kazakova S M, Serebrijski I G
Abstract excerpt
A pULB113 (RP4::mini-Mu cts) plasmid was used to generate a library of prime plasmids carrying fragments of the Methylobacillus flagellatum genome. The genes carried by these prime plasmids were identified by complementation after transfer to suitably marked Escherichia coli and Pseudomonas aerug...
Topics
- Chromosome Mapping
- Chromosomes, Bacterial
- Conjugation, Genetic
- Crosses, Genetic
- Escherichia coli
- Gene Library
- Genetic Complementation Test
- Genetic Markers
- Methylococcaceae
- Mutation
- Plasmids
