Increased expression of the bifunctional protein PrlF suppresses overproduction lethality associated with exported beta-galactosidase hybrid proteins in Escherichia coli.

Abstract excerpt

We have cloned and determined the nucleotide sequence of the prlF gene. An open reading frame predicting a 111-amino-acid protein (Mr 12,351) with an acidic carboxy terminus was identified. The DNA sequence preceding this open reading frame revealed a putative promoter and a ribosome-binding site...

Topics

• Bacterial Proteins
• Base Sequence
• Biological Transport
• Cloning, Molecular
• Escherichia coli
• Galactosidases
• Genes, Bacterial
• Homeostasis
• Molecular Sequence Data
• Mutation
• Phenotype
• beta-Galactosidase