Co-overexpression of prlF increases cell viability and enzyme yields in recombinant Escherichia coli expressing Bacillus stearothermophilus alpha-amylase.

doi:10.1021/bp00034a007

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Article

Co-overexpression of prlF increases cell viability and enzyme yields in recombinant Escherichia coli expressing Bacillus stearothermophilus alpha-amylase.

Biotechnology progress - 1 Jan 2000

Minas W, Bailey J E

Abstract excerpt

The effects on cloned amylase production of co-overexpression of prlF, a gene that appears to interact with the sec protein export machinery in Escherichia coli, was investigated by comparing three expression systems: (i) a high copy number plasmid with the Bacillus stearothermophilus alpha-amyla...

Topics

Endopeptidases
Escherichia coli
Fermentation
Gene Expression
Geobacillus stearothermophilus
Mutation
Plasmids
Recombinant Proteins
alpha-Amylases

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