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Article

Cloning, characterization, and DNA sequence of the rfaLK region for lipopolysaccharide synthesis in Salmonella typhimurium LT2.

Journal of bacteriology - 1 Nov 1991

MacLachlan P R, Kadam S K, Sanderson K E

Abstract excerpt

We have cloned and sequenced the rfaL and rfaK genes for lipopolysaccharide synthesis in Salmonella typhimurium LT2 on a 4.28-kb HindIII fragment from the previously described R' factor pKZ3 (S. K. Kadam, A. Rehemtulla, and K. E. Sanderson, J. Bacteriol. 161:277-284, 1985). rfaL is thought to encode a component of the O-antigen ligase, and rfaK is believed to encode the N-acetylglucosamine transferase. The genes...

Topics

Amino Acid Sequence
Base Sequence
Blotting, Southern
Carbohydrate Sequence
Cloning, Molecular
DNA Transposable Elements
DNA, Bacterial
Escherichia coli
Genes, Bacterial
Genetic Complementation Test

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Genotype

Lipopolysaccharides

Molecular Sequence Data

Mutagenesis, Insertional

Open Reading Frames

Operon

Protein Conformation

Restriction Mapping

Source

Open in PubMed Open full text

Identifiers

PMID: 1657881
DOI: 10.1128/jb.173.22.7151-7163.1991
PMCID: PMC209221

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1657881-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Cloning, characterization, and DNA sequence of the rfaLK region for lipopolysaccharide synthesis in Salmonella typhimurium LT2.PMID 1657881Read ↗

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