Article
Improved ZAP-70 assay using two clones, multiple methods of analysis and clinical correlation.
Cytometry. Part B, Clinical cytometry - 1 Sept 2011
Degheidy Heba A, Venzon David J, Farooqui Mohammed Z H, Abbasi Fatima, Arthur Diane C, Wilson Wyndham H, Wiestner Adrian, Stetler-Stevenson M A, Marti Gerald E
Abstract excerpt
INTRODUCTION: In a companion methodological study, we compared two anti-ZAP-70 clones (1E7.2 AF 488 and SBZAP PE) and four selected methods of analysis. Clinical correlations are required for validation. METHODS: Multicolor flow-cytometric evaluation of ZAP-70, CD38, CD69, CD26, CD49d, and CD27 was tested in 45 untreated-CLL patients. Four methods of ZAP-70 expression analysis and a scoring system were designed....
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