Article
ZAP-70 by flow cytometry: a comparison of different antibodies, anticoagulants, and methods of analysis.
Cytometry. Part B, Clinical cytometry - 15 Jul 2006
Best O G, Ibbotson R E, Parker A E, Davis Z A, Orchard J A, Oscier D G
Abstract excerpt
BACKGROUND: The clinical course of chronic lymphocytic leukaemia (CLL) is variable. ZAP-70 expression is believed to provide prognostic information. The flow cytometric detection of ZAP-70 is difficult because it is an intracellular antigen with weak expression in CLL. Consensus has not been reached as to the best method for measurement. METHODS: We analyzed 72 CLL patient samples for ZAP-70 expression and IgVH...
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