ZAP-70 by flow cytometry: a comparison of different antibodies, anticoagulants, and methods of analysis.

doi:10.1002/cyto.b.20121

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Article

ZAP-70 by flow cytometry: a comparison of different antibodies, anticoagulants, and methods of analysis.

Cytometry. Part B, Clinical cytometry - 15 Jul 2006

Best O G, Ibbotson R E, Parker A E, Davis Z A, Orchard J A, Oscier D G

Abstract excerpt

BACKGROUND: The clinical course of chronic lymphocytic leukaemia (CLL) is variable. ZAP-70 expression is believed to provide prognostic information. The flow cytometric detection of ZAP-70 is difficult because it is an intracellular antigen with weak expression in CLL. Consensus has not been reached as to the best method for measurement. METHODS: We analyzed 72 CLL patient samples for ZAP-70 expression and IgVH...

Topics

Antibodies
Anticoagulants
Antigen-Antibody Reactions
Biomarkers, Tumor
Disease Progression
Flow Cytometry
Humans
Immunoglobulin Heavy Chains
Immunoglobulin Variable Region
Leukemia, Lymphocytic, Chronic, B-Cell

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Middle Aged

Mutation

Reproducibility of Results

Sensitivity and Specificity

Staining and Labeling

Time Factors

ZAP-70 Protein-Tyrosine Kinase

Source

Open in PubMed

Identifiers

PMID: 16906573
DOI: 10.1002/cyto.b.20121

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