Article
Genome-scale reconstruction of metabolic network in Bacillus subtilis based on high-throughput phenotyping and gene essentiality data.
The Journal of biological chemistry - 28 Sept 2007
Oh You-Kwan, Palsson Bernhard O, Park Sung M, Schilling Christophe H, Mahadevan Radhakrishnan
Abstract excerpt
In this report, a genome-scale reconstruction of Bacillus subtilis metabolism and its iterative development based on the combination of genomic, biochemical, and physiological information and high-throughput phenotyping experiments is presented. The initial reconstruction was converted into an in silico model and expanded in a four-step iterative fashion. First, network gap analysis was used to identify 48...
Topics
- Bacillus subtilis
- Bacterial Proteins
- Computer Simulation
- Genome, Bacterial
- Models, Biological
- Phenotype