Article
Cleavage-site preferences of Sindbis virus polyproteins containing the non-structural proteinase. Evidence for temporal regulation of polyprotein processing in vivo.
The EMBO journal - 1 Aug 1990
de Groot R J, Hardy W R, Shirako Y, Strauss J H
Abstract excerpt
The non-structural proteins of Sindbis virus, nsP1, 2, 3 and 4, are produced upon cleavage of polyproteins P123 and P1234 by a proteinase residing in nsP2. We used cell free translation of SP6 transcripts to study the proteolytic activity of nsP2 and of nsP2-containing polyproteins. To generate p...
Topics
- Animals
- Base Sequence
- Capsid
- Cells, Cultured
- Chick Embryo
- Codon
- Endopeptidases
- Fibroblasts
- Gene Expression Regulation, Viral
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Plasmids
- Polymerase Chain Reaction
- Protein Biosynthesis