Regulation of Sindbis virus RNA replication: uncleaved P123 and nsP4 function in minus-strand RNA synthesis, whereas cleaved products from P123 are required for efficient plus-strand RNA synthesis.

Abstract excerpt

Nonstructural proteins of Sindbis virus, nsP1, nsP2, nsP3, and nsP4, as well as intermediate polyproteins, are produced from two precursor polyproteins, P123 and P1234, by a proteolytic enzyme encoded in the C-terminal half of nsP2. We studied the requirements for and the functions of the intermediate and mature processing products for Sindbis virus RNA synthesis by using site-directed mutants which have a...

Topics

• Animals
• Base Sequence
• Biological Evolution
• Cell-Free System
• Cells, Cultured
• Chickens
• Cricetinae
• DNA-Directed RNA Polymerases
• Gene Expression
• Genetic Complementation Test
• Molecular Sequence Data