Article
Cleavage between nsP1 and nsP2 initiates the processing pathway of Sindbis virus nonstructural polyprotein P123.
Virology - 1 Jul 1990
Shirako Y, Strauss J H
Abstract excerpt
The cleavage between nsP1 and nsP2 and that between nsP2 and nsP3 in the Sindbis virus nonstructural polyproteins was studied with respect to order of processing and enzyme-substrate relationships, using site-specific mutants in which the cleavage sites had been altered. The penultimate Gly in nsP1 or nsP2 or both was substituted by Ala, Val, or Glu, and processing was studied in vitro. Substitution with Ala...
Topics
- Amino Acid Sequence
- Base Sequence
- Capsid
- Chromosome Deletion
- Cloning, Molecular
- Coliphages
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Plasmids
- Promoter Regions, Genetic