Article
Use of the fluorescence activated cell sorter to enrich dendritic cells from mouse spleen.
Journal of immunological methods - 4 Oct 1990
Crowley M T, Inaba K, Witmer-Pack M D, Gezelter S, Steinman R M
Abstract excerpt
Dendritic cells are a specialized but trace population of antigen presenting cells that always have been enriched by multi-step procedures over a period of 1 or more days in tissue culture. Here we describe the isolation of dendritic cells from fresh mouse spleen suspensions using the FACS and a...
Topics
- Animals
- Antibodies, Monoclonal
- Antigen-Presenting Cells
- Cell Separation
- Cell Survival
- Cytokines
- Dendritic Cells
- Female
- Flow Cytometry
- Integrins
- Islets of Langerhans
- Lymphocyte Culture Test, Mixed
- Male
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Phenotype
- Spleen