Article
Use of the fluorescence activated cell sorter to enrich dendritic cells from mouse spleen.
Journal of immunological methods - 4 Oct 1990
Crowley M T, Inaba K, Witmer-Pack M D, Gezelter S, Steinman R M
Abstract excerpt
Dendritic cells are a specialized but trace population of antigen presenting cells that always have been enriched by multi-step procedures over a period of 1 or more days in tissue culture. Here we describe the isolation of dendritic cells from fresh mouse spleen suspensions using the FACS and a monoclonal antibody, N418, to the p150/90 member of the leukocyte integrin family (Metlay et al., 1990). By two color...
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