Article
In vivo kinetics and characterization of IFN-gamma-producing cells during a thymus-independent immune response.
Journal of immunology (Baltimore, Md. : 1950) - 15 Jul 1991
van den Eertwegh A J, Fasbender M J, Schellekens M M, van Oudenaren A, Boersma W J, Claassen E
Abstract excerpt
Using immunohistochemical techniques, we studied IFN-gamma-producing cells (IFN-gamma-PC) in vivo during immune responses to thymus-independent type-2 (TI-2) Ag. Detection of IFN-gamma-PC in cryostat sections of spleen-tissue was performed with an enzyme labeled mAb directed against IFN-gamma. Af...
Topics
- Animals
- Antibody Formation
- Antibody-Producing Cells
- Antigens, T-Independent
- Ficoll
- Immunoenzyme Techniques
- Immunoglobulin M
- Interferon-gamma
- Mice
- Mice, Inbred BALB C
- Phenotype
- Spleen
- T-Lymphocytes
- Time Factors
- Trinitrobenzenes
