Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome.

Genome research - 1 Jun 2011

Alsford Sam, Turner Daniel J, Obado Samson O, Sanchez-Flores Alejandro, Glover Lucy, Berriman Matthew, Hertz-Fowler Christiane, Horn David

Abstract excerpt

African trypanosomes are major pathogens of humans and livestock and represent a model for studies of unusual protozoal biology. We describe a high-throughput phenotyping approach termed RNA interference (RNAi) target sequencing, or RIT-seq that, using Illumina sequencing, maps fitness-costs associated with RNAi. We scored the abundance of >90,000 integrated RNAi targets recovered from trypanosome libraries...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Computational Biology
Share to Gene Library

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genetic Fitness

Share to Genome, Protozoan

Share to High-Throughput Nucleotide Sequencing

Share to Plasmids

Share to RNA Interference

Share to Trypanosoma brucei brucei

Source

Open in PubMed Open full text

Identifiers

PMID: 21363968
DOI: 10.1101/gr.115089.110
PMCID: PMC3106324

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome.PMID 21363968Read ↗

Genome-scale RNAi screens for high-throughput phenotyping in bloodstream-form African trypanosomes.Semantic proximity Read ↗

Genome-scale functional profiling of cell cycle controls in African trypanosomesSemantic proximity Read ↗

A platform for robust quantitative functional genomics reveals temporal fitness landscapes in African trypanosomesSemantic proximity Read ↗

Chromosome-wide analysis of gene function by RNA interference in the african trypanosome.Semantic proximity Read ↗

Genome-scale RNA interference profiling of Trypanosoma brucei cell cycle progression defectsSemantic proximity Read ↗

A <i>Trypanosoma brucei</i> ORFeome-based Gain-of-Function Library identifies genes that promote survival during melarsoprol treatmentSemantic proximity Read ↗

Genome-wide RNAi screens in bloodstream form trypanosomes identify drug transportersSemantic proximity Read ↗

Regulators of Trypanosoma brucei Cell Cycle Progression and Differentiation Identified Using a Kinome-Wide RNAi ScreenSemantic proximity Read ↗

Flagellar motility is required for the viability of the bloodstream trypanosome.Semantic proximity Read ↗

Transcriptomic analysis of N-terminal mutated <i>Trypanosoma cruzi</i> UBP1 knockdown underlines the importance of this RNA-binding protein in parasite developmentSemantic proximity Read ↗

Identification of positive and negative regulators in the stepwise developmental progression towards infectivity in Trypanosoma bruceiSemantic proximity Read ↗

Transcriptomic analysis of N-terminal mutated Trypanosoma cruzi UBP1 knockdown underlines the importance of this RNA-binding protein in parasite development.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.