Article
Real-time RNA profiling within a single bacterium.
Proceedings of the National Academy of Sciences of the United States of America - 28 Jun 2005
Le Thuc T, Harlepp Sébastien, Guet Calin C, Dittmar Kimberly, Emonet Thierry, Pan Tao, Cluzel Philippe
Abstract excerpt
Characterizing the dynamics of specific RNA levels requires real-time RNA profiling in a single cell. We show that the combination of a synthetic modular genetic system with fluorescence correlation spectroscopy allows us to directly measure in real time the activity of any specific promoter in prokaryotes. Using a simple inducible gene expression system, we found that induced RNA levels within a single bacterium...
Topics
- Bacterial Proteins
- Cell Cycle
- Cell Proliferation
- Dose-Response Relationship, Drug
- Drug Resistance, Multiple
- Escherichia coli
- Escherichia coli Proteins
- Gene Expression Regulation, Bacterial
- Genes, Reporter
- Genotype
- Green Fluorescent Proteins
- Models, Statistical