Construction and genetic analysis of murine hepatitis virus strain A59 Nsp16 temperature sensitive mutant and the revertant virus.

doi:10.1007/s12250-011-3145-x

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Article

Construction and genetic analysis of murine hepatitis virus strain A59 Nsp16 temperature sensitive mutant and the revertant virus.

Virologica Sinica - 1 Feb 2011

Chang Guo-hui, Luo Bao-Jun, Lu Pin, Lin Lei, Wu Xiao-yan, Li Jing, Hu Yi, Zhu Qing-yu

Abstract excerpt

Coronaviruses (CoVs) are generally associated with respiratory and enteric infections and have long been recognized as important pathogens of livestock and companion animals. Mouse hepatitis virus (MHV) is a widely studied model system for Coronavirus replication and pathogenesis. In this study, we created a MHV-A59 temperature sensitive (ts) mutant Wu"-ts18(cd) using the recombinant vaccinia reverse genetics...

Topics

Amino Acid Substitution
Animals
Cell Line
Mice
Murine hepatitis virus
Mutation
Reverse Transcriptase Polymerase Chain Reaction
Temperature
Viral Proteins
Virus Replication

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Identifiers

PMID: 21331887
DOI: 10.1007/s12250-011-3145-x
PMCID: PMC7091325

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