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Article

Murine hepatitis virus nsp4 N258T mutants are not temperature-sensitive.

Virology - 20 Jan 2013

Beachboard Dia C, Lu Xiaotao, Baker Susan C, Denison Mark R

Abstract excerpt

Coronavirus replicase nsp4 is critical for virus-induced membrane modifications. An nsp4 mutant (N258T) of murine hepatitis virus (MHV) has been reported to be temperature-sensitive (ts) and to alter membrane targeting. We engineered and recovered all four possible codon variants of N258T in the cloned MHV-A59 background. All mutant viruses demonstrated impaired replication compared to wildtype MHV, but no nsp4...

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Identifiers

PMID: 23099203
DOI: 10.1016/j.virol.2012.10.001
PMCID: PMC3804408

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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