Article
A Single Point Mutation, Asn16→Lys, Dictates the Temperature-Sensitivity of the Reovirus tsG453 Mutant.
Viruses - 12 Feb 2021
Glover Kathleen K M, Sutherland Danica M, Dermody Terence S, Coombs Kevin M
Abstract excerpt
Studies of conditionally lethal mutants can help delineate the structure-function relationships of biomolecules. Temperature-sensitive (ts) mammalian reovirus (MRV) mutants were isolated and characterized many years ago. Two of the most well-defined MRV ts mutants are tsC447, which contains mutations in the S2 gene encoding viral core protein σ2, and tsG453, which contains mutations in the S4 gene encoding major...
Topics
- Amino Acid Substitution
- Capsid
- Capsid Proteins
- Humans
- Mammalian orthoreovirus 3
- Phenotype
- Point Mutation
- Reoviridae Infections
- Temperature