Article
Biotinylated probe isolation of targeted gene region improves detection of T790M epidermal growth factor receptor mutation via peptide nucleic acid-enriched real-time PCR.
Clinical chemistry - 1 May 2011
Li Jin, Jänne Pasi A, Makrigiorgos G Mike
Abstract excerpt
BACKGROUND: The presence of the EGFR (epidermal growth factor receptor) T790M mutation in tumor tissue or body fluids from patients treated with EGFR tyrosine kinase inhibitors may indicate the onset of resistance to treatment. It is important to identify this mutation as early as possible so that treatment can be modified accordingly or potential side effects of further treatment can be avoided. This requirement...
Topics
- Biotin
- Cell Line, Tumor
- DNA
- Drug Resistance, Neoplasm
- ErbB Receptors
- Humans
- Limit of Detection
- Lung Neoplasms
- Male
- Molecular Probes
- Mutation
- Oligonucleotides
- Peptide Nucleic Acids
- Polymerase Chain Reaction