Article
Chemically modulating the photophysics of the GFP chromophore.
The journal of physical chemistry. B - 17 Feb 2011
Conyard Jamie, Kondo Minako, Heisler Ismael A, Jones Garth, Baldridge Anthony, Tolbert Laren M, Solntsev Kyril M, Meech Stephen R
Abstract excerpt
There is growing interest in engineering the properties of fluorescent proteins through modifications to the chromophore structure utilizing mutagenesis with either natural or unnatural amino acids. This entails an understanding of the photophysical and photochemical properties of the modified chromophore. In this work, a range of GFP chromophores with different alkyl substituents are synthesized and their...
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