Fluorescent derivatives of the GFP chromophore give a new insight into the GFP fluorescence process.

doi:10.1016/S0006-3495(03)74612-8

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Article

Fluorescent derivatives of the GFP chromophore give a new insight into the GFP fluorescence process.

Biophysical journal - 1 Sept 2003

Follenius-Wund Anny, Bourotte Maryline, Schmitt Martine, Iyice Fatih, Lami Hans, Bourguignon Jean-Jacques, Haiech Jacques, Pigault Claire

Abstract excerpt

The photophysical properties of synthetic compounds derived from the imidazolidinone chromophore of the green fluorescent protein were determined. Various electron-withdrawing or electron-donating substituents were introduced to mimic the effect of the chromophore surroundings in the protein. The absorption and emission spectra as well as the fluorescence quantum yields in dioxane and glycerol were shown to be...

Topics

Biophysical Phenomena
Biophysics
Dioxanes
Fluorescent Dyes
Glycerol
Green Fluorescent Proteins
Hydrogen Bonding
Imidazoles
Kinetics
Light
Luminescent Proteins
Microscopy, Fluorescence
Models, Chemical

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Identifiers

PMID: 12944297
DOI: 10.1016/S0006-3495(03)74612-8
PMCID: PMC1303356

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