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Article

BRAFV600E detection in melanoma is highly improved by COLD-PCR.

Clinica chimica acta; international journal of clinical chemistry - 12 May 2011

Pinzani Pamela, Santucci Claudio, Mancini Irene, Simi Lisa, Salvianti Francesca, Pratesi Nicola, Massi Daniela, De Giorgi Vincenzo, Pazzagli Mario, Orlando Claudio

Abstract excerpt

BACKGROUND: The BRAF gene has been identified as an oncogene in human cancer and the V600E mutation has been shown to be associated with clinico pathological features of primary invasive melanomas. As BRAF may be an attractive therapeutic target, it is crucial to have a sensitive method for detec...

Topics

Adult
Aged
Aged, 80 and over
DNA Mutational Analysis
Female
Formaldehyde
Humans
Male
Melanoma
Middle Aged
Mutation
Nucleic Acid Denaturation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Paraffin Embedding

Polymerase Chain Reaction

Proto-Oncogene Proteins B-raf

Transition Temperature

Source

Identifiers

PMID: 21262211
DOI: 10.1016/j.cca.2011.01.014

Genes

BRAF

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-21262211-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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