Article
Multiplexed shotgun genotyping for rapid and efficient genetic mapping.
Genome research - 1 Apr 2011
Andolfatto Peter, Davison Dan, Erezyilmaz Deniz, Hu Tina T, Mast Joshua, Sunayama-Morita Tomoko, Stern David L
Abstract excerpt
We present a new approach to genotyping based on multiplexed shotgun sequencing that can identify recombination breakpoints in a large number of individuals simultaneously at a resolution sufficient for most mapping purposes, such as quantitative trait locus (QTL) mapping and mapping of induced mutations. We first describe a simple library construction protocol that uses just 10 ng of genomic DNA per individual...
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