Article
Rapid SNP discovery and genetic mapping using sequenced RAD markers.
PloS one - 1 Jan 2008
Baird Nathan A, Etter Paul D, Atwood Tressa S, Currey Mark C, Shiver Anthony L, Lewis Zachary A, Selker Eric U, Cresko William A, Johnson Eric A
Abstract excerpt
Single nucleotide polymorphism (SNP) discovery and genotyping are essential to genetic mapping. There remains a need for a simple, inexpensive platform that allows high-density SNP discovery and genotyping in large populations. Here we describe the sequencing of restriction-site associated DNA (RAD) tags, which identified more than 13,000 SNPs, and mapped three traits in two model organisms, using less than half...
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