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Article

A two-hybrid assay to study protein interactions within the secretory pathway.

PloS one - 28 Dec 2010

Dube Danielle H, Li Bin, Greenblatt Ethan J, Nimer Sadeieh, Raymond Amanda K, Kohler Jennifer J

Abstract excerpt

Interactions of transcriptional activators are difficult to study using transcription-based two-hybrid assays due to potent activation resulting in false positives. Here we report the development of the Golgi two-hybrid (G2H), a method that interrogates protein interactions within the Golgi, where transcriptional activators can be assayed with negligible background. The G2H relies on cell surface glycosylation to...

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Identifiers

PMID: 21209940
DOI: 10.1371/journal.pone.0015648
PMCID: PMC3011011

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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