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Article

Development and application of a DNA microarray-based yeast two-hybrid system.

Nucleic acids research - 1 Feb 2013

Suter Bernhard, Fontaine Jean-Fred, Yildirimman Reha, Raskó Tamás, Schaefer Martin H, Rasche Axel, Porras Pablo, Vázquez-Álvarez Blanca M, Russ Jenny, Rau Kirstin, Foulle Raphaele, Zenkner Martina, Saar Kathrin, Herwig Ralf, Andrade-Navarro Miguel A, Wanker Erich E

Abstract excerpt

The yeast two-hybrid (Y2H) system is the most widely applied methodology for systematic protein-protein interaction (PPI) screening and the generation of comprehensive interaction networks. We developed a novel Y2H interaction screening procedure using DNA microarrays for high-throughput quantitative PPI detection. Applying a global pooling and selection scheme to a large collection of human open reading frames,...

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Identifiers

PMID: 23275563
DOI: 10.1093/nar/gks1329
PMCID: PMC3561971

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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