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Structural and functional analysis of temperature-sensitive mutants of the phage phi 29 DNA polymerase.

Nucleic acids research - 25 Aug 1990

Blasco M A, Blanco L, Parés E, Salas M, Bernad A

Abstract excerpt

The cloning and complete sequencing of gene 2 from four independently isolated temperature-sensitive mutants in the phage phi 29 DNA polymerase (ts2 mutants) is reported. The results obtained indicate that, in vivo, the mutations only affect the initial steps of the replication process. Interestingly, three of these mutations consist in the single amino acid change Ala to Val at position 492 of the protein. The...

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Identifiers

PMID: 2118623
PMCID: PMC331939

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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