Article
Structural and functional analysis of temperature-sensitive mutants of the phage phi 29 DNA polymerase.
Nucleic acids research - 25 Aug 1990
Blasco M A, Blanco L, Parés E, Salas M, Bernad A
Abstract excerpt
The cloning and complete sequencing of gene 2 from four independently isolated temperature-sensitive mutants in the phage phi 29 DNA polymerase (ts2 mutants) is reported. The results obtained indicate that, in vivo, the mutations only affect the initial steps of the replication process. Interesti...
Topics
- Amino Acid Sequence
- Bacillus subtilis
- Bacteriophages
- Cloning, Molecular
- DNA Replication
- DNA-Directed DNA Polymerase
- Gene Expression Regulation, Viral
- Genes, Viral
- Metals
- Molecular Sequence Data
- Mutation
- Phenotype
- Sequence Homology, Nucleic Acid
- Temperature
- Virus Replication