Prevalence of temperature sensitive folding mutations in the parallel beta coil domain of the phage P22 tailspike endorhamnosidase.

Abstract excerpt

Temperature sensitive mutations fall into two general classes: tl mutations, which render the mature protein thermolabile, and tsf (temperature sensitive folding) mutations, which destabilize an intermediate in the folding pathway without altering the functions of the folded state. The molecular defects caused by tsf mutations have been intensively studied for the elongated tailspike endorhamnosidase of...

Topics

• Bacteriophage P22
• Glycoside Hydrolases
• Hot Temperature
• Mutation
• Protein Binding
• Protein Folding
• Viral Tail Proteins