Article
Structure of a fluorescent protein from Aequorea victoria bearing the obligate-monomer mutation A206K.
Acta crystallographica. Section F, Structural biology and crystallization communications - 1 Aug 2012
von Stetten David, Noirclerc-Savoye Marjolaine, Goedhart Joachim, Gadella Theodorus W J, Royant Antoine
Abstract excerpt
The green fluorescent protein (GFP) from the jellyfish Aequoria victoria has been shown to dimerize at high concentrations, which could lead to artefacts in imaging experiments. To ensure a truly monomeric state, an A206K mutation has been introduced into most of its widely used variants, with minimal effect on the spectroscopic properties. Here, the first structure of one of these variants, the cyan fluorescent...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.