Article
Hetero-oligomeric tagging diminishes non-specific aggregation of target proteins fused with Anthozoa fluorescent proteins.
The Biochemical journal - 1 Apr 2003
Bulina Maria E, Verkhusha Vladislav V, Staroverov Dmitry B, Chudakov Dmitry M, Lukyanov Konstantin A
Abstract excerpt
The tendency for tetramerization is the main disadvantage in the green fluorescent protein homologues from Anthozoa species. We report a universal method called hetero-oligomeric tagging, which diminishes troublesome consequences of tetramerization of Anthozoa-derived fluorescent proteins (FP) in intracellular protein labelling. This approach is based on the co-expression of the FP-tagged protein of interest...
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