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Article

Mapping and Quantitation of the Interaction between the Recombination Activating Gene Proteins RAG1 and RAG2.

The Journal of biological chemistry - 8 May 2015

Zhang Yu-Hang, Shetty Keerthi, Surleac Marius D, Petrescu Andrei J, Schatz David G

Abstract excerpt

The RAG endonuclease consists of RAG1, which contains the active site for DNA cleavage, and RAG2, an accessory factor whose interaction with RAG1 is critical for catalytic function. How RAG2 activates RAG1 is not understood. Here, we used biolayer interferometry and pulldown assays to identify regions of RAG1 necessary for interaction with RAG2 and to measure the RAG1-RAG2 binding affinity (KD ∼0.4 μM) (where...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Open in PubMed Open full text

Identifiers

PMID: 25745109
DOI: 10.1074/jbc.M115.638627
PMCID: PMC4424321

Genes

RAG1
RAG2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Mapping and Quantitation of the Interaction between the Recombination Activating Gene Proteins RAG1 and RAG2.PMID 25745109Read ↗

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