Excretion into the culture medium of a Bacillus beta-glucanase after overproduction in Escherichia coli.

doi:10.1515/znc-1990-3-416

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Article

Excretion into the culture medium of a Bacillus beta-glucanase after overproduction in Escherichia coli.

Zeitschrift fur Naturforschung. C, Journal of biosciences - 1 Jan 2000

Riethdorf S, Ulrich A, Völker U, Hecker M

Abstract excerpt

The beta-glucanase gene (bgl) from Bacillus amyloliquefaciens was expressed in E. coli CSH 55 under the control of the PR promoter of phage lambda that is repressed by the thermosensitive repressor C1857. Production of beta-glucanase was drastically stimulated by a temperature shift to 42 degrees...

Topics

Bacillus
Escherichia coli
Genes, Bacterial
Genotype
Glucans
Glucosidases
Kinetics
Molecular Weight
Plasmids
Recombinant Proteins
Restriction Mapping
beta-Glucosidase

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