Heterologous Kluyveromyces lactis beta-galactosidase production and release by Saccharomyces cerevisiae osmotic-remedial thermosensitive autolytic mutants.

doi:10.1016/s0304-4165(97)00048-2

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Article

Heterologous Kluyveromyces lactis beta-galactosidase production and release by Saccharomyces cerevisiae osmotic-remedial thermosensitive autolytic mutants.

Biochimica et biophysica acta - 6 Jun 1997

Becerra M, Cerdán E, González Siso M I

Abstract excerpt

The beta-galactosidase from Kluyveromyces lactis is a high molecular weight protein with commercial interest. A major drawback of its industrial production is the high cost associated with extraction and downstream processing due to its intracellular nature. In this work, the effectiveness of the...

Topics

Cell Wall
Hot Temperature
Kluyveromyces
Mutation
Plasmids
Saccharomyces cerevisiae
beta-Galactosidase

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